The colony formation assay revealed that cell colony formation efficiency were lower in PKCε siRNA-transfected cells than in control siRNA-transfected and untransfected cells [(29.6 ± 1.4)% vs. (60.9 ± 1.5)% and (50.9 ± 1.1)%, P < 0.05], suggesting that PKCε may be important for the growth and survival of RCC cells. The gene discussed is PRKCE; the disease is renal cell carcinoma.