Here, we analyzed the PINK1-KO mouse model by a two-dimensional difference gel electrophoresis (2D-DIGE) based approach to determine protein expression alterations in three distinct brain regions: the midbrain, which is the location of substantia nigra, the striatum, which is the major efferent region of substantia nigra, and the cerebral cortex, which is more distal, and thus less relevant to PD pathology. This evidence concerns the gene PINK1 and Parkinson disease.