Doxorubicin specifically inhibited TGF-β-signaling in human lung adenocarcinoma A549 cells by blocking TGF-β1-induced activation of SMAD3-responsive CAGA12-Luc reporter while other drugs like Cisplatin or Methotrexate did not alter activation of CAGA12-Luc reporter under the same conditions suggesting that that such inhibitory effect could be a novel mechanism of Doxorubicin action towards tumor cells [96]. This evidence concerns the gene SMAD3 and neoplasm.