In a different study, human metastatic melanoma cells were treated with siRNA against BRAFV600E and S-phase kinase-associated protein-2 (Skp-2), a positive regulator of RhoA, which resulted in both cell migration and invasion inhibition, suggesting that the BRAF-MAPK pathway and Skp-2-RhoA cascade can contribute to the invasive nature of melanoma [45]. The gene discussed is RHOA; the disease is melanoma.