siRNA-mediated MYC depletion in 4 human prostate cancer cell lines was followed by quantitative real-time PCR to measure the expression of the mature miR-26a, its 2 primary forms, mir-26a1, and mir-26a2, as well as the host genes in which they are embedded, CTDSPL and CTDSP2. Additionally, we measured the expression of the mature and primary forms of miR-26b, and CTDSP1, the gene which harbors miR-26b. The gene discussed is CTDSP2; the disease is prostate cancer.