Our findings demonstrate that high NF-κB and low Notch1 expression are correlated with high expression of VEGFR-3 (a marker of LVD) and VEGF-C, in ESCC patients, revealing an inverse relationship between Notch1 and NF-κB signaling and tumor-induced lymphangiogenesis. This evidence concerns the gene NOTCH1 and esophageal squamous cell carcinoma.