AKT1 and neoplasm: Further analysis also revealed that phosphorylated membrane and cytoplasmic IRS-1 Y612-positive tumours (median HScore cutoff of 61) expressed higher levels of phosphorylated membrane IGF-IR Y1316 (P = 0.011 (n = 50)), phosphorylated membrane and cytoplasmic Akt (P ≤ 0.001 (n = 50)) and nuclear Ki-67 (P = 0.022 (n = 40)) immunostaining than did phosphorylated membrane and cytoplasmic IRS-1 Y612-negative tumours (not shown).