CD276 and malignant colon neoplasm: Western blot analysis was performed to determine the molecular weight of sB7-H3 and a band near 37 kDa was detected in human 2IgB7-H3- and mouse B7-H3-transfected cell supernatants (Fig. 3B and Fig. 3C), as well as in lung cancer, uterine cervix cancer and colon cancer cell line supernatants (data not shown).