Western blot analysis was performed to determine the molecular weight of sB7-H3 and a band near 37 kDa was detected in human 2IgB7-H3- and mouse B7-H3-transfected cell supernatants (Fig. 3B and Fig. 3C), as well as in lung cancer, uterine cervix cancer and colon cancer cell line supernatants (data not shown). This evidence concerns the gene CD276 and colonic neoplasm.