G6PD and metabolic disease: PK (M1/M2) is located on gene locus 15q22 composed of 12 exons spanning 32 kb [27] mainly found in striated muscle, brain, fetus, leukocytes, platelets, lungs, spleen, kidneys, adipose tissue etc. In vivo and in vitro studies on the function of nsSNPs have found that genetic mutations in G6PD and PK genes are responsible for RBC metabolism disorders [28]–[38].