Similar to the results obtained with LCMV cl-13, infection of both LCMV WE54 and WE22 was significantly reduced in cells depleted of Hrs, Tsg101, Vps22, Vps24, and Alix (Fig. 9B), suggesting that α-DG dependent and α-DG independent LCMV isolates pass through the MVB and require the ESCRT machinery for cell entry. This evidence concerns the gene PDCD6IP and infection.