As shown in Figure 3B (lower panel), exposure to MI-319 increased the nuclear pool of p53 in SKMEL5-GSK-3βS9A cells and the addition of sorafenib induced its disappearance from the nucleus and translocation to the mitochondria, similar to what was observed in melanoma cell lines with high constitutive GSK-3β activity such as A375. Here, TP53 is linked to melanoma.