This microarray chip allows the simultaneous detection of nine loci of four deafness-associated genes, namely, 35delG, 176-191del16, 235delC and 299-300delAT for GJB2 gene, 538C > T for GJB3 gene, 1555A > G and 1494C > T for mtDNA 12S rRNA gene, and 2168A > G (H723R) and IVS7-2A > G for SLC26A4 gene. Here, GJB3 is linked to deafness.