AHSG and breast cancer: To show that the release of exosomes for adhesion is not unique to BT-549-Gal3, we also treated MDA-MB-231, another popular breast cancer cell line without (control) and with 30 μM of BAPTA-AM for 1 h and demonstrated that whereas the control untreated cells adhered to fetuin-A coated wells after detachment, the cells pre-treated with BAPTA-AM failed to do so after detachment and only did so in the presence of added serum exosomes (125 μg/ml) (Fig. 5C).