No significant differences in MFI of these costimulatory molecules were observed using the four different maturation cocktails (sample size: CD80/CD86/CD273/CD274 in HC: C: n = 10/10/4/8, CP: n = 16/16/3/7, R: n = 13/11/4/13, RP: n = 11/9/3/10; in AML patients: C: n = 10/10/4/9, CP: n = 14/14/3/10, R: n = 10/7/4/10, RP: n = 8/6/3/6). Here, CD274 is linked to acute myeloid leukemia.