Levels of inflammatory markers, such as C-reactive protein, tumor necrosis factor (TNF) and interleukin 6 (IL-6), increase with age and obesity, and decrease accordingly with CR [81], and the mediators of this decline are various, including the already mentioned Hsp70, PGC-1α and neurotrophic factors such as BDNF, but here we will focus on two key proteins involved which are SIRT1 and mTOR. This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.