Considering that memory T-cell responses might be critical for protection against HIV-1 infection [30], [31], [32], [33], we assessed by IFN-γ ELISPOT and IFN-γ and IL-2 intracellular cytokine staining (ICS) the long-term immunogenicity profile (i.e. 53 days after the boost) elicited by DNA-B/MVA-B and DNA-B/MVA-B ΔC6L vaccination in splenocytes. This evidence concerns the gene IL2 and HIV-1 infection.