Given the fact that bFGF can activate the STAT3 pathway in many cell types, we investigated in this study whether the antitumor effects of Ad-bFGF-siRNA correlate with the reduced activation of the STAT3 signaling pathway to further our current understanding of the underlying mechanisms of Ad-bFGF-siRNA-induced growth suppression and apoptosis of glioma cells. The gene discussed is FGF2; the disease is central nervous system cancer.