MAP1LC3A and infection: In addition, the induction of the autophagic process evidenced microscopically as the punctate LC3 pattern was also observed when HeLa, MEF or T24 cells transiently expressing ERFP-LC3 were challenged with Serratia following the same infection protocol as used for CHO-EGFP-LC3 cells without addition of gentamicin (Fig. 3, panels a–d and g–h).