Previously, Kasinski et al. reported a mechanism by which EF24 suppressed the NF-κB signaling pathway through direct action on the I-κB kinase (IKK) in many cancer cell types including human epithelial cervical (HeLa), breast (MDA-MB-231), human prostate (PC3), colon (HT29), human lung (A549, A460, Calu-1), non-small cell lung (H157, H358) and ovarian (1A9) cells[30]. This evidence concerns the gene NFKB1 and cancer.