TP53 and hepatocellular carcinoma: We used five HCC cell lines with different p53 status (HepG2 and SK-HEP-1 with wt p53; PLC/PRF/5 and Huh 7 with p53 mutation; Hep3B with null p53) which are widely used in the liver cancer research to investigate the effects of EF24 on HCC.