Our results clearly indicate that the pharmacological inhibitors of ERα, c-Src, PI3K and mTOR not only inhibited Akt/mTOR pathway (Figure 5A), but also inhibited the expression of HIF-1α (Figure 6A), confirming the importance of ERα/c-Src/PI3K assembly in the non-genomic signal transduction of E2 in breast cancer lines. The gene discussed is SRC; the disease is breast cancer.