When a cut-off for positivity was defined at >10%, the proportion of DCIS showing positive staining for the different tumor markers were as follows: ER (62.4%), PR (55.6%), HER2/neu (31.6%), MIB1 (39.8%), p53 (22.6%), p21 (39.8%), Cyclin D1 (95.5%) calgranulin (20.5%), psoriasin (12%). This evidence concerns the gene ERBB2 and ductal breast carcinoma in situ.