INSR and hepatocellular carcinoma: In contrast to our hypothesis, however, not all genes are down-regulated after cell suffering from BBR treatment in previous studies, including pgp-170 in human hepatoma cell lines [30], gata-3 and gata-2 in 3T3L1 cell lines [31], insR in type 2 diabetes animal models [32], wee1 in leukemia cells [33], and ldlr in hepatic cells [34], etc. All of the promoters in these genes lack the canonical TATA box sequence.