To further understand the effect of CypE on the replication and/or transcription of influenza A virus, three types of RNA levels (viral RNA (vRNA), complementary RNA (cRNA), and mRNA) of the NP and M1 genes were detected with or without CypE knock-down by quantitative real-time PCR 4 and 8 h after viral infection. Here, PPIE is linked to viral infectious disease.