Consistent with these findings, analysis of the distribution of staining intensity scores (Figure 1C) showed that while the proportion of staining scores was similar between normal and DCIS subgroups, Bit1 staining frequencies varied dramatically between the normal/DCIS and the invasive node-negative/node-positive subgroups with the node-positive tumor tissues exhibiting the highest frequency of reduced Bit1 immunoreactivity. The gene discussed is PTRH2; the disease is ductal breast carcinoma in situ.