We found that reovirus infection alone caused activation of NF-κB transcription in both p53+/+ and p53−/− cells using a dual-luciferase assay, whereas Nutlin-3a alone had negligible effect on the activation of NF-κB (Figure 5A and C and Supplementary Figure S4A and B). Here, TP53 is linked to Reoviridae infectious disease.