To further explore the effects of PGE2 on activation of JNK1/2 in human LoVo colon cancer cells, we treated LoVo cells with PGE2 (10-6M) for various time periods (15 min, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h), and subsequently measured the phosphorylation/activation of proteins by immunoblotting assay. The gene discussed is MAPK8; the disease is malignant colon neoplasm.