CD4 and infection: A pronounced increase in the proportion of CD4+FoxP3+Tregs within colonic granulomas at the chronic phase of infection, from 2.9±0.6% to 18.8±0.7% was revealed by enumeration of double positive versus single positive cells in anti-CD4 / anti-FoxP3 immunostained cryosections of colonic tissue (Fig. 3D & E).