These results indicate that spontaneous IFN-γ-producing CD8+ T cell responses against wild-type p53 were not detectable even in seropositive cancer patients by our presensitization protocol in contrast to the frequent detection of NY-ESO-1-specific CD8+ T cell responses in NY-ESO-1-seropositive patients (Figure S1(A)). The gene discussed is IFNG; the disease is cancer.