In contrast to the undetectable p53-specific CD8+ T cells, significant IFN-γ-producing p53-specific CD4+ T cell responses to subpools of overlapping peptides were found in 6 out of 12 seropositive patients (Figure 3A and data not shown), with comparable magnitude to spontaneous CD4+ T cell responses against NY-ESO-1 in NY-ESO-1 seropositive ovarian cancer patients in this study cohort (Figure S1(B)). This evidence concerns the gene CD4 and ovarian carcinoma.