To this end, we analyzed by immunofluorescence and quantified the level of expression of two β-catenin/TCF target genes (Ccnd1/Cyclin D1 and Lef1) in colon ACF and carcinomas of Apcmin/+Vdr+/+, Apcmin/+Vdr+/- and Apcmin/+Vdr-/- mice. Here, LEF1 is linked to carcinoma.