We have described that 1,25(OH)2D3 and several less calcemic analogs interfere the Wnt/β-catenin pathway in a series of human colon cancer cell lines in several modes: they increase the binding of VDR to β-catenin hampering the formation of β-catenin/TCF complexes, induce the expression of the Wnt inhibitor DKK1, and promote the relocation of β-catenin from the nucleus towards the plasma membrane where it binds E-cadherin at adherens junctions[13], [14]. This evidence concerns the gene HNF4A and malignant colon neoplasm.