FOXP3 and colitis: To further directly quantitate the methylated/demethylated status of Foxp3 and IL-17 gene promoters during colitis , we performed real-time PCR using sets of forward and reverse primers and Taqman probes and using bisulfite converted genomic DNA from MLN or LP cells of the following groups of mice: Vehicle alone, TCDD alone, DSS+vehicle, and DSS+TCDD.