PLAAT4 and infection: Fig. 1A shows that empty vector-infected cells increase in number over 72 h, but that cell number is significantly reduced at 48 and 72 h in TIG3-expressing cells. Fig. 1B shows that TIG3 level is maximal in the infected cells by 24 and 48 h post-infection and is reduced by 72 h. In addition to the TIG3 monomer, we observe accumulation of high molecular weight forms which are thought to be covalently-crosslinked TIG3 [10]–[12]. As previously reported, TIG3 is expressed at low levels in most transformed cells [10], [11] and therefore is not detected at time zero.