The result of this study of the SP molecular markers indicated generally that the prevalence of the triple mutation indicated by dhfr Arg-59 was relatively low, 5%, whereas the dhps Glu-540 was very rare suggesting that the selection process had not reached dhps. However, eight years have passed since the study, and in this area where the people are aware that CQ is no longer effective in treating malaria and where SP is readily available even over the counter in pharmacies further selection of dhfr and dhps mutations might have rapidly taken place. The gene discussed is DHFR; the disease is malaria.