In this study, we have used multiplex PCR combined with next generation sequencing of pooled DNA, to explore the mutation burden in DISC1 and 10 of its interaction partners (ATF5, GRB2, FEZ1, LIS-1 (encoded by PAFAH1B1), PDE4B, NDE1, NDEL1, TRAF3IP1, YWHAE, and ZNF365) in schizophrenia patients versus control individuals. The gene discussed is PDE4B; the disease is schizophrenia.