To evaluate GR-mediated gene transactivation in influenza-infected vs mock-infected cells, 293T cells were cotransfected with a GR expressing plasmid, a reporter plasmid containing the Firefly-luciferase gene under the control of a minimal TK promoter and GR-responsive elements, and a Renilla-luciferase expressing construct. The gene discussed is NR3C1; the disease is influenza.