To demonstrate if VEGFR-2 knockdown under conditions of hSulf-1 overexpression has the same effect on cell viability, BEL-7404 cancer cells, which were infected with Ad5-hSulf1 at an MOI of 10 pfu/cell, were transfected with VEGFR-2 shRNA vector at a concentration of 20 μg/105 cells to knockdown the expression of VEGFR-2 (Fig. S1), the results showed that BEL-7404 cell viability after transfection of VEGFR-2 shRNA was further decreased in the context of hSulf-1 effect (Fig. 3C). The gene discussed is SULF1; the disease is cancer.