To evaluate the role of conjugated Herceptin via two independent linkers, both wild-type Herceptin and Herceptin conjugates (20 μg/mL) were incubated with two independent Her2-positive cell lines, colon cancer line OS187 and osteosarcoma line CCH.OS.D (Figure 1c). The gene discussed is ERBB2; the disease is colonic neoplasm.