ERBB2 and neoplasm: The result confirmed our hypothesis because Nec-1 treatment reversed the cell survival reduction by Herceptin conjugate treatment as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Figure 6c), which suggested that necrosis was the crucial mechanism in EDC-Herceptin conjugate-induced Her2-positive tumor cell death.