AKT1 and neoplasm: BT474 tumours excised (Figure 5A) were lysed and protein extracts were analysed by western blotting to detect tyrosine phosphorylated ErbB-2 (PY1248), total ErbB-2, phosphorylated ERK1/2, total ERK1/2, phosphorylated AKT1 and total AKT1 proteins.