To examine the downstream targets of Nm23 involved in alcohol induced cell invasion, we determined the effects of Nm23 overexpression and 0.5% v/v ethanol treatment on 84 genes associated with extracellular matrix regulation and adhesion molecules in the following groups of breast cancer cells: 1) T47D controls cells (empty vector), 2) T47D cells treated with 0.5% v/v alcohol (empty vector), 3) T47D cells overexpressing Nm23, and 4) T47D cells overexpressing Nm23 and treated with 0.5% alcohol. This evidence concerns the gene NME1 and breast cancer.