(Fig. S11) We propose that these findings have implications for the role of LGR5 in colorectal cancer progression: induction of wnt activity by APC mutation or β-catenin mutation would maintain LGR5 expression outside the stem cell niche, however overstimulation of wnt signalling at the invasive front of a tumour (where there is loss of E-cadherin and β-catenin is reported to be strongly nuclear: [65]) would be expected to reduce LGR5 expression, thus facilitating wnt-stimulated EMT and favouring invasiveness. This evidence concerns the gene APC and colorectal cancer.