Since the natural route of infection for Salmonella is via the oral route we also determined the impact of infection with S. Typhimurium C5507 Vi+ and SGB1 Vi− on intracellular production of MIP-2, TNF-α, IFN-γ and perforin by immune cells of the MLNs 24 h post inoculation. The gene discussed is TNF; the disease is infection.