Young ovarian TILs that had been expanded directly from fresh enzyme-digested tumor specimens exhibited autologous tumor reactivity ex vivo (IFN-g secretion >200 pg/mL and twice background) that was statistically similar to the reactivity of TILs that had been outgrown in parallel IL-2 cultures (p = 0.95; n = 4; Figure 5h). This evidence concerns the gene IL2 and neoplasm.