We tested the SV40 promoter as a strong promoter that was inserted into the pGL SV40 plasmid, and we used the minimal promoter of the human GLI3 gene (NM_000168; Greig cephalopolysyndactyly syndrome; MIM #175700) as an example of a relatively weak promoter [20]. The gene discussed is GLI3; the disease is Greig cephalopolysyndactyly syndrome.