AECs were also isolated from naïve Balb/c or TLR-9-/- mice, and these cells were infected with either 0.01 or 0.001 MOI (multiplicity of infection) of γHV68 for 48 hours, before cell lysates were prepared and analyzed for cleaved caspase 3 by western blotting. The gene discussed is TLR9; the disease is infection.