To validate the expression stability of CASC3, EIF2B1, IPO8, MRPL19, PGK1, POP4, PPIA, RPL37A plus GAPDH and ACTB, thirty-four randomly chosen primary cultured meningiomas, the meningioma cell line IOMM-Lee, two arachnoidea, six dura mater, one cerebral meninges and two pooled normal brain samples were screened for these reference genes. The gene discussed is EIF2B1; the disease is meningioma.