CD133 expression (qRT-PCR - Figure 3A) and methylation of its promoter (PYRO 1 assay - Figure 3B-D) were then measured in primary epithelial cultures derived from clinical samples of benign prostatic hyperplasia (BPH), CaP and castration resistant prostate cancer (CR-CaP). The gene discussed is PROM1; the disease is benign prostatic hyperplasia.