While RNF146 RNAi can inhibit autocrine Wnt signaling in teratocarcinoma cells and also stabilize tankyrase proteins in colorectal cancer cells with APC mutation-driven signaling, we find that RNF146 knockdown does not significantly affect Wnt signaling or Axin protein stabilization in the colorectal cell lines tested. Here, TNKS is linked to colorectal cancer.