While RNF146 RNAi can inhibit autocrine Wnt signaling in teratocarcinoma cells and also stabilize tankyrase proteins in colorectal cancer cells with APC mutation-driven signaling, we find that RNF146 knockdown does not significantly affect Wnt signaling or Axin protein stabilization in the colorectal cell lines tested. This evidence concerns the gene TNKS and teratocarcinoma.