The NAFLD activity score (NAS) of ApoC3Tg mice was ≍4 (Fig. 1B) after HFD feeding, and NAS of ≥5 has been correlated with a diagnosis of NASH.15 In contrast, there were no significant differences in plasma concentrations of interleukin (IL)-1β, IL-10, IL-12p70, IL-8, and IL-6 levels (Supporting Fig. 2A) or hepatic nuclear factor kappaB (NF-κB) p65, IkappaBα, or c-Jun N-terminal kinase (JNK) phosphorylation (Supporting Fig. 2A,B) between genotypes fed the HFD. This evidence concerns the gene MAPK8 and metabolic dysfunction-associated steatotic liver disease.