To investigate whether down-regulation of SLC19A3 expression is associated with hypermethylation of SLC19A3 promoter CpG islands, we performed methylation-specific qPCR on those 15 pairs of primary breast cancer tumor tissues and their adjacent non-tumor breast tissues using primers targeting on a hypermethylated promoter CpG region as previously studied [10]. Here, SLC19A3 is linked to neoplasm.