Intranasal administration of live BCG before OVA challenge suppressed IL-5 production and airway eosinophilia in an IFN-γ dependent manner [8], while subcutaneous treatment with killed M. vaccae before OVA sensitization inhibited allergic airway inflammation through the induction of a CD4+CD45RBlo regulatory T cell secreted IL-10 and TGF-β [42]. This evidence concerns the gene TGFB1 and Increased total eosinophil count.