In the Nontreated Ischemic Retinas and DMSO-Treated Ischemic Retinas; GFAP was significantly upregulated in the ILM, GCL, IPL, and INL with clear staining at Müller cell processes, and was overly expressed due to the damage to the retina by ischemia after vascular pruning, which indicates glial reactivity. This evidence concerns the gene GFAP and ischemia.